Obtention of tetanus antitoxin hyperimmune sera in horses

UltramicroELISA for measuring tetanus antitoxin in human sera.

This article describes a combination of methods--a solid-phase enzyme-linked immunosorbent assay (ELISA) combined with an ultramicroanalytical system (UMAS)--that can be used to measure tetanus antitoxin activity in human sera or plasma. The test, which is rapid and permits analysis of 78 samples of serum per reaction plate with a volume of 10 microL of diluted serum per sample, is proposed as ...

Measurement of Epsilon Antitoxin in Sheep Sera by ELISA

Human tetanus antitoxin.

Tetanus Antitoxin from Intraocular Transplants **

Evidence for antibody formation by various cells, tissues, and organs has come mainly from studies employing the following methods: (i) extracts of tissue cultures,'l (ii) cannulization of afferent and efferent lymph,"'X (iii) extracts of cells, tissues, and organs,"' (iv) extirpation of the spleen," and (v) roentgen-radiation of animals with lead-protected mobilized spleens.' Although the repo...

Rheumatoid Factor Properties of Hyperimmune Rabbit Sera

The rheumatoid factor-like substance (RFLS) induced by hyperimmunization of rabbits with bacteria was consistently and exclusively associated with the macroglobulin fraction of the sera studied. The RFLS is separable from the bulk of serum proteins by zone centrifugation, DEAE cellulose chromatography, and gel filtration. Partially purified preparations of the RFLS were studied by immunoelectro...